Principles of a quantitative assay for bacterial endotoxins in blood that uses Limulus lysate and a chromogenic substrate

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Principles of a quantitative assay for bacterial endotoxins in blood that uses Limulus lysate and a chromogenic substrate.

Some factors affecting the use of chromogenic substrates with Limulus lysate for assaying bacterial endotoxins in blood have been assessed. It was found that endogenous amidases, which degrade the substrate, could be inactivated by heating serum at 60 degrees C for 15 min. Endotoxin was found not to be removed from serum during clotting. A potent inhibitor of the activated lysate was found to b...

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Determination of endotoxin in injectable antibiotic preparations by the chromogenic assay method using a Limulus reagent (Tachypleus hemocyte lysate) and a chromogenic substrate.

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1980

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.12.5.644-650.1980